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Image Search Results
Journal: PLOS One
Article Title: Evaluation of bone regenerative and bioactive potential of β-TCP/PLLA/PGA nanobiomaterial in conjunction with concentrated conditioned media from rapidly expanding clone (REC) of mesenchymal stem cells: A pilot animal study
doi: 10.1371/journal.pone.0332531
Figure Lengend Snippet: (A) LepR expression in the β-TCP/PLLA/PGA–plain media, β-TCP/PLLA/PGA–1 × REC-CM, and β-TCP/PLLA/PGA–10 × REC-CM groups at week 1 (×4 magnification, scale bar = 200 µm; × 20 magnification, scale bar = 100 µm; × 40 magnification, scale bar = 50 µm). (B) LepR expression in the β-TCP/PLLA/PGA–plain media, β-TCP/PLLA/PGA–1 × REC-CM, and β-TCP/PLLA/PGA–10 × REC-CM groups at week 4 (×4 magnification, scale bar = 200 µm; × 20 magnification, scale bar = 100 µm; × 40 magnification, scale bar = 50 µm). (C) At both time points, the β-TCP/PLLA/PGA–10 × REC-CM group showed significant differences from the other groups (**p < 0.0002; *p < 0.05).
Article Snippet: Primary antibody incubation was performed at room temperature for 50 min using the following specific antibodies: anti-Runx2 rabbit polyclonal antibody (1:300; Abcam: ab23981), anti-human osteocalcin monoclonal antibody clone (1:3000; BIO-RAD 0400−0041),
Techniques: Expressing
Journal: Cell Death & Disease
Article Title: Elevated fatty acid β-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway
doi: 10.1038/s41419-023-05641-2
Figure Lengend Snippet: A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without anti-leptin antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of leptin receptor was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Article Snippet: Incubate with
Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Control
Journal: Cell Death & Disease
Article Title: Elevated fatty acid β-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway
doi: 10.1038/s41419-023-05641-2
Figure Lengend Snippet: A Protein–protein interactions (PPI) among leptin, leptin receptor and major metabolic pathways was consturcted. Only the PPI with the confidence of >0.4 was kept. B AMPK total and phosphorylated protein at T172 (pAMPK), LKB1 and CAMKK protein levels were compared in RA-FLS treated in the presence or absence of leptin by western blot ( n = 4). C Assessment of LKB1 protein level in RA-FLS processed with vehicle, anti-leptin antibody and isotype antibody by western blot ( n = 4). D AMPK total and pAMPK in NC or down-regulation of LKB1 with siRNA in the presence or absence of leptin was detected at 48 h ( n = 4). E CPT-1A gene ( n = 5) and protein ( n = 4) levels in RA-FLS stimulated by leptin with or without compound C (CC, 5 μM) was evaluated. Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, *** P < 0.001, **** P < 0.0001, ns means no significance compared with control.
Article Snippet: Incubate with
Techniques: Protein-Protein interactions, Western Blot, Control